Method of treating pain associated with a cartilage defect

A method of treating pain associated with a cartilage defect in a joint, the method comprising:

• applying an effective amount of a preparation to said cartilage defect in a subject, wherein the preparation is the product of the process comprising:
• (a) collecting an amnion sample from a donor;
• (b) rinsing the amnion sample to remove blood clots that may be present but to preserve growth factors that may be present, to produce a rinsed amnion;
• (c) freezing the rinsed amnion to produce a frozen amnion;
• (d) cryomilling the frozen amnion to produce a micronized amnion;
• (e) collecting an amniotic fluid sample from the donor;
• (f) separating a cellular fraction comprising viable amniotic fluid cells from the amniotic fluid sample;
• (g) combining the cellular fraction with the micronized amnion to produce a combined amnion/amniotic fluid cell mixture;
• and
• (h) suspending the combined amnion/amniotic fluid cell mixture in a biocompatible carrier;
• wherein the effective amount is effective to reduce the pain associated with the cartilage defect in the joint.