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US 2026/0069637 A1Published application
Compositions and methods for repairing cartilage defects
AssigneeVericel Corporation
InventorsRoss A. Tubo, Xing Zhao
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods for repairing cartilage defects.
Claims21
-24. (canceled)
A method of preparing a cell bank, the method comprising the steps of:
- obtaining a tissue sample from a human;
- inspecting the tissue sample for contamination;
- weighing the tissue sample;
- mincing the tissue sample;
- digesting the tissue sample;
- counting cells in the tissue sample to determine viability;
- culturing the cells;
- and
- cryogenically freezing the cells for storage.
6 more elements
A method of characterizing a cell bank sample, the method comprising steps of:
- thawing cryogenically frozen primary cell bank samples;
- culturing secondary samples from the primary samples;
- culturing tertiary samples from the secondary samples;
- and
- assaying the samples to determine cell viability, mycoplasma, endotoxin, sterility, senescence, identity, and/or aggrecan values.
2 more elements
A method of culturing a cell bank sample, the method comprising steps of:
- thawing a cryogenically frozen cell bank sample;
- adding culture medium to a flask;
- counting cells to determine viability;
- growing the cells and the medium in a cell culture;
- feeding the cell culture;
- and
- treating the cell culture with 0.05% trypsin solution until the cells are detached from the flask.
4 more elements
Dependencies are inferred from the claim text.