Reagents for Directed Biomarker Signal Amplification

A conjugated polymer comprising n-conjugated repeat units, wherein the 71-conjugated repeat units comprise:

• an Ar polycyclic repeat unit that is evenly or randomly distributed along the polymer main chain;
• an MU polymer modifying unit that is evenly or randomly distributed along the polymer main chain;
• and
• a linker L1 that is an aryl or a heteroaryl group evenly or randomly distributed along the polymer main chain;
• and
• G1 and G2 are terminating groups that are each independently selected hydrogen, halogen, amine, carbamate, carboxylic acid, maleimide, activated esters, N-hydroxysuccinimidyl, hydrazines, hydrazids, hydrazones, azide, alkyne, aldehydes, thiol, optionally substituted aryl, optionally substituted heteroaryl, halogen substituted aryl, boronic acid substituted aryl, boronic ester substituted aryl, boronic esters, boronic acids, optionally substituted fluorene and aryl or heteroaryl, wherein G1 and G2 are different and do not comprise a repeat unit.

A conjugated polymer comprising n-conjugated repeat units, wherein the n-conjugated repeat units comprise:

• an Ar polycyclic repeat unit that is evenly or randomly distributed along the polymer main chain;
• an MU polymer modifying unit that is evenly or randomly distributed along the polymer main chain;
• and
• a linker L1 that is an aryl or a heteroaryl group evenly or randomly distributed along the polymer main chain;
• G1 which is a first terminating group and is independently selected hydrogen, halogen, amine, carbamate, carboxylic acid, maleimide, activated esters, N-hydroxysuccinimidyl, hydrazines, hydrazids, hydrazones, azide, alkyne, aldehydes, thiol, optionally substituted aryl, optionally substituted heteroaryl, halogen substituted aryl, boronic acid substituted aryl, boronic ester substituted aryl, boronic esters, boronic acids, optionally substituted fluorene and aryl or heteroaryl;
• and
• G2 which is a second terminating group and comprises aryl or heteroaryl substituted with a pendant chain terminated with:
• a functional group selected from amine, carbamate, carboxylic acid, carboxylate, maleimide, activated esters, N-hydroxysuccinimidyl, hydrazines, hydrazids, hydrazones, azide, alkyne, aldehydes, thiols, and protected groups thereof for conjugation to another substrate, molecule or biomolecule;
• or
• a biomolecule.