Compositions for and methods of detecting food toxins
AssigneesThe Rgents of The University of California, The Board of Trustees of the Leland Stanford Junior University
InventorsDaniel L. MINOR, Justin DU BOIS, Lauren O'CONNELL, Zhou CHEN, Sandra ZAKRZEWSKA, Fayal ABDEREMANE-ALI, Aurora ALVAREZ-BUYLLA
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Abstract
The present disclosure is directed, in part, to saxiphilin proteins, nucleic acids encoding the same, compositions comprising the same, kits comprising the same, and methods of detecting toxin in samples. In some embodiments, the toxin is saxitoxin or derivatives of the same.
Claims66
A system comprising:
- (i) a solid support comprising at least one vessel, wherein the vessel comprises a solution comprising a first amino acid sequence that comprises at least about 70% sequence identity to X1X2X3X4X5X6X7X8X9X10X11X12X13X14;
- or
- (ii) a solid support comprising a solid surface upon which the first amino acid sequence is absorbed, bound covalently or bound non-covalently, wherein the first amino acid sequence comprises at least about 70% sequence identity to X1X2X3X4X5X6X7X8X9X10X11X12X13X14;
- wherein X1=I, V, F, L
- X2=any amino acid
- X3=F
- X4=D
- X5=any amino acid
- X6=M, Q, I
- X7=any amino acid
- X8=R, K
- X9=any amino acid
- X10=any amino acid
- X11=any amino acid
- X12=any amino acid
- X13=D
- X14=Y, V
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A method of detecting the quantity or presence of a paralytic shellfish poisoning (PSP) toxin in a sample, the method comprising:
- (i) exposing the sample to a composition comprising an amino acid sequence having at least 90% sequence identity to least 90% sequence identity to X1X2X3X4X5X6X7X8X9X10X11X12X13X14;
- wherein X1=I, V, F, L
- X2=any amino acid
- X3=F
- X4=D
- X5=any amino acid
- X6=M, Q, I
- X7=any amino acid
- X8=R, K
- X9=any amino acid
- X10=any amino acid
- X11=any amino acid
- X12=any amino acid
- X13=D
- X14=Y, V
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A method of assaying the toxicity of a subject comprising:
- (i) exposing a sample from the subject to a composition comprising an amino acid sequence having at least 70% sequence identity to X1X2X3X4X5X6X7X8X9X10X11X12X13X14;
- wherein X1=I, V, F, L
- X2=any amino acid
- X3=F
- X4=D
- X5=any amino acid
- X6=M, Q, I
- X7=any amino acid
- X8=R, K
- X9=any amino acid
- X10=any amino acid
- X11=any amino acid
- X12=any amino acid
- X13=D
- X14=Y, V;
- (ii) measuring an association between the amino acid sequence and a paralytic shellfish poisoning (PSP) toxin;
- and
- (iii) determining that the subject comprises a toxic substance if the association between the amino acid sequence and the PSP toxin is higher than a threshold level of toxin;
- or determining that the subject is free of a toxic substance if the association between the amino acid sequence and the PSP toxin is lower than the threshold level of the toxin.
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The method of, wherein the PSP toxin is a STX or TTX.
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Dependencies are inferred from the claim text.