Methods and compositions for flow cytometer calibration
OwnerLorena Hidalgo San Jose, Aaron Jacob Tyznik +1inventors
InventorsLorena Hidalgo San Jose, Aaron Jacob Tyznik, Stephanie J. Widmann-Nguyen
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OwnerLorena Hidalgo San Jose, Aaron Jacob Tyznik +1inventors
InventorsLorena Hidalgo San Jose, Aaron Jacob Tyznik, Stephanie J. Widmann-Nguyen
Estimating the term…
The present disclosure provides improved and useful techniques for cross-standardization of flow cytometry instruments and, particularly, spectral flow cytometry instruments. Aspects of the disclosure include methods of calibrating a flow cytometer having a plurality of fluorescence channels. Methods of interest utilize calibration sets of bead populations, wherein each bead population of the calibration set includes a different fluorophore attached to a surface thereof and the calibration set includes a number of bead populations that is less than the number of fluorescence channels of the flow cytometer. Flow cytometers, non-transitory computer-readable storage media, and kits including, e.g., calibration sets of bead populations for carrying out the subject methods are also provided.
A method of calibrating a flow cytometer comprising a plurality of fluorescence channels, the method comprising:
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Dependencies are inferred from the claim text.