Flow cell devices and use thereof

A method for preparing a flow cell for DNA sequencing reactions, comprising:

• (a) providing the flow cell comprising (i) an inlet and an outlet, wherein the inlet comprises an open landing area for receiving one or more reagents, and (ii) one or more channels disposed between the inlet and the outlet for performing the sequencing reactions;
• (b) openly dispensing a first reagent of the one or more reagents to the open landing area to flow at least part of the first reagent from the open landing area to the one or more channels;
• (c) introducing a gas into the one or more channels;
• (d) openly dispensing a second reagent of the one or more reagents to the open landing area to flow at least part of the second reagent from the open landing area to the one or more channels, thereby removing a residual amount of the first reagent from the one or more channels.

A method for sequencing with a flow cell device, comprising:

• (a) providing the flow cell comprising (i) an inlet and an outlet, wherein the inlet comprises an open landing area for receiving one or more reagents, and (ii) one or more channels disposed between the inlet and the outlet for performing the sequencing reactions;
• (b) openly dispensing a first reagent of the one or more reagents to the open landing area to flow at least part of the first reagent from the open landing area to the one or more channels;
• (c) removing a residual amount of the first reagent from at least part of the open landing area by flowing the residual amount of the first reagent through a cleaning outlet of the flow cell device;
• and
• (d) openly dispensing a second reagent of the one or more reagents to the open landing area to flow at least part of the second reagent from the open landing area to the one or more channels.

A method for manufacturing a flow cell device, comprising:

• obtaining one or more substrates;
• generating one or more channels in the one or more substrates;
• forming an inlet comprising a hole in one of the one or more substrates and an open landing area, wherein the inlet is in fluidic connection with the one or more channels;
• forming an outlet that is in fluidic connection with the one or more channels;
• coating at least a portion of a surface of the one or more channels with a first coating;
• and
• fixedly coupling the one of one or more substrates together.