MUTANT WICKERHAMMYCES CIFERRll STRAIN HAVING IMPROVED PRODUCTIVITY FOR TAPS, AND METHOD FOR PRODUCING TAPS USING SAME
AssigneeKorea University Research and Business Foundation
InventorsMin-Kyu OH, Seok Woo YOO
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Abstract
The present invention relates to a Wickerhamomyces ciferrii mutant strain having an improved ability to produce tetraacetylphytosphingosine (TAPS), and a method for producing TAPS using the same. The Wickerhamomyces ciferrii mutant strain provided by the present invention exhibits excellent TAPS productivity, and thus may be widely used in development of various products that use TAPS.
Claims19
An expression plasmid for genetic engineering of Wickerhamomyces ciferrii, containing a CEN/ARS origin of replication and a uracil selection marker.
A Wickerhamomyces ciferrii strain having an improved ability to produce tetraacetylphytosphingosine (TAPS), obtained by enhancing activities of serine palmitoyl-transferase and sphinganine C4-hydroxylase in a uracil auxotrophic Wickerhamomyces ciferrii strain containing a uracil selectable marker using an expression plasmid containing a CEN/ARS origin of replication and further weakening an activity of a long-chain base kinase.
A method for constructing a Wickerhamomyces ciferrii strain having an improved ability to produce tetraacetylphytosphingosine (TAPS), comprising:
- a first step of constructing a uracil auxotrophic Wickerhamomyces ciferrii strain using 5-FOA;
- a second step of enhancing activities of serine palmitoyl-transferase and sphinganine C4-hydrosylase using an expression plasmid containing a CEN/ARS origin of replication;
- and
- a third step of weakening an activity of a long-chain base kinase.
Dependencies are inferred from the claim text.