Method and device for treating an adipose tissue
OwnerMaria Cristina COLLIVIGNARELLI, Andrea POGGIO +1inventors
InventorsMaria Cristina COLLIVIGNARELLI, Andrea POGGIO, Antonello TATEO
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Abstract
The invention concerns a method, and a device that can implement this method, adapted to obtain adipose based compounds, of selectively variable particle sizes, distinguished by high levels of purity and by high concentrations of viable mesenchymal stem cells, which can be used in autologous transplantation procedures mainly for regenerative purposes, as well as for filling and/or volumizing purposes.
Claims13
Method for treating an adipose tissue comprising the step of:
- collecting an adipose tissue (T1) from a donor patient, characterized in that comprises the steps of:
- providing a plurality of sieves with progressively decreasing mesh sizes;
- providing radial stirrer means of oscillating type;
- providing vacuum dehydration-filter means;
- subjecting said adipose tissue (T1) to a first physical-mechanical treatment step by means of said plurality of sieves with progressively decreasing mesh size and by means of said radial stirrer means of oscillating type, separating at least an oversize fraction containing fibrous fractions of different particle sizes and a remaining wet and homogenized undersize fraction, of Nanofat type, rich in stem cells;
- subjecting at least said remaining wet and homogenized undersize fraction to a second physical treatment step, by means of a vacuum dehydration-filter treatment, to eliminate the water, in order to concentrate the same fraction and obtain an adipose tissue (T2a) of Nanofat particle size, without fibrous and/or oleic and/or haematic residues (R1) and aqueous residues (R2), containing viable mesenchymal stem cells in a high concentration, where said adipose tissue (T2a) is adapted to be destined for reimplantation in the same donor patient.
Device for treating an adipose tissue (T1), collected from a donor patient, characterized in that it comprises:
- a filtering column (1), provided with at least a first and a second sieve (3, 4), with mesh of decreasing size according to the direction of the filtering flow, adapted to be operated by radial stirrer means (5) of oscillating type;
- a vacuum dehydration-filter apparatus (2), provided with a filter (8), adapted to be operated by a suction pump (9),
- where said filtering column (1), by means of said second sieve (4), allows an undersize (T2a) and aqueous residue (R2) and comprising a wet adipose tissue of Nanofat particle size and an oversize comprising an adipose tissue (T2b) of Microfat particle size, naturally with a low water content, to be obtained and where said vacuum dehydration-filter apparatus (2) allows an adipose tissue (T2a) without aqueous residues (R2) to be obtained, where each adipose tissue (T2a), (T2b) is without fibrous and/or oleic and/or haematic residues (R1), contains viable mesenchymal stem cells in a high concentration, and at least one of these is destined for reimplantation in the same donor patient.
Dependencies are inferred from the claim text.