Hydrophobic interaction chromatography (hic) composition and method of producing the hic composition
OwnerBarry Edward Boyesinventor
Life of this patent
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Abstract
A hydrophobic interaction chromatography (HIC) composition includes a solid phase substrate and a hydrophobic-modified hydrophilic ligand covalently coupled to the solid phase substrate. The hydrophobic-modified ligand includes a hydrophilic ligand portion covalently bonded to the solid phase substrate with the hydrophilic ligand portion including a polar group and a plurality of hydroxyl groups. The hydrophobic-modified ligand also includes a peptide segment covalently coupled to the hydrophilic ligand portion and comprising from two to twenty amino acid residues. The peptide segment is linearly arranged, and each amino acid is the same as or different than the other amino acid residues for promoting HIC interaction.
Claims39
A hydrophobic interaction chromatography (HIC) composition comprising:
- a solid phase substrate;
- and
- a hydrophobic-modified hydrophilic ligand covalently coupled to the solid phase substrate with the hydrophobic-modified ligand comprising;
- a hydrophilic ligand portion covalently bonded to the solid phase substrate with the hydrophilic ligand portion including a polar group and a plurality of hydroxyl groups, and
- a peptide segment, the peptide segment directly or indirectly covalently coupled to the hydrophilic ligand portion and comprising from two to twenty amino acid residues, including natural or non-natural amino acid residues,
- wherein the peptide segment is linearly arranged and wherein each amino acid residue is the same as or different than the other amino acid residues for promoting HIC interactions.
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A method of producing a hydrophobic interaction chromatography (HIC) composition including a hydrophobic-modified hydrophilic ligand, the method comprising:
- providing a solid phase substrate;
- providing a hydrophilic ligand portion including a polar group and a plurality of hydroxyl groups;
- reacting the solid phase substrate and the hydrophilic ligand portion to covalently couple the hydrophilic ligand portion to the solid phase substrate to form a hydrophilic-modified substrate;
- providing an activation compound including a leaving group;
- reacting the activation compound with one of the plurality of hydroxyl groups to form an activated hydrophilic-modified substrate;
- providing a peptide segment comprising from two to twenty amino acid residues, including natural or non-natural amino acid residues;
- and
- reacting the activated hydrophilic-modified substrate with the peptide segment to release the leaving group of the activation compound and form the hydrophobic-modified hydrophilic ligand and the HIC composition;
- wherein the peptide segment is linearly arranged and each amino acid residue is the same as or different than the other amino acid residues for promoting HIC interaction.
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Dependencies are inferred from the claim text.