Method for obtaining a porous injectable scaffold based on similar biopolymers, but with different melting temperatures

Method for generating a porous injectable scaffold as a biological matrix which includes the stages of:

• i) providing a liquid composition of 2 phases at a temperature below 25° C., of Newtonian behavior, where the composition comprises:
• a first liquid dispersant phase at room temperature formed by a gelatin with a low melting point, less than 15° C. determined by the fact that it has a proline and hydroxyproline content of 18% or less with respect to the total amino acid content;
• which is additionally functionalized with methacryloyl or methacrylamide groups;
• and a photoinitiator;
• and a second dispersed phase, of solid-state microdroplets or beads of a gelatin solution with a melting point greater than 25° C., determined by having a proline and hydroxyproline content of 20% or more of the total amino acid content;
• (ii) extruding the solution provided at the site where the porous scaffold will be formed:
• (iii) initiating the polymerization of the liquid dispersant phase by applying light radiation;
• (iv) raising the temperature to 35-40° C. and allow melting of the beads of the dispersed phase;
• and
• v) obtaining a porous scaffold by jellification of the dispersing phase and discarding by melting and/or diffusion of the dispersed phase.