Mineralization of cell-laden matrices
AssigneeOregon Health & Science University
InventorLuiz E. Bertassoni
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Abstract
This disclosure relates to methods of mineralizing cell-laden matrices. Disclosed herein are cell-laden matrix compositions. Also disclosed herein are methods of selectively mineralizing a cell-laden matrix. Methods of culturing biomimetic bone tissue are disclosed herein. Also disclosed herein are kits containing compositions disclosed herein or portions thereof.
Claims29
A three-dimensional (3D) cell-encapsulated matrix composition comprising:
- (a) a mineralizing solution, the mineralizing solution being supersaturated with respect to one or more crystallizable metals and having a pH from about 6.0 to about 8.0, in which the crystallizable metals comprise alkali metals, earth alkali metals, or both, including ionic calcium, ionic phosphorus, or both;
- (b) a buffering agent having a pH buffering range of about 6.0 to about 8.0;
- (c) living cells;
- (d) a 3D cell-encapsulated scaffold comprising hydrogel with the living cells configured to undergo directed mineralization to mimic native bone nanoscale structure;
- (e) a basal medium comprising nutrients, buffering agents, and growth factors for supporting the growth of the living cells;
- and
- (f) a nucleation inhibitor in an amount effective to modulate ions of the crystallizable metals, reducing cytotoxic effects on the living cells by substantially controlling against spontaneous precipitation.
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A method of selectively mineralizing a three-dimensional (3D) cell-encapsulated matrix comprising:
- providing a 3D cell-encapsulated matrix having a 3D cell-capsulated hydrogel scaffold with the living cells configured to undergo directed mineralization to mimic native bone nanoscale structure;
- providing a mineralizing solution comprising:
- a supersaturated solution with respect to one or more crystallizable metals in which the crystallizable metals comprise alkali metals, earth alkali metals, or both, including ionic calcium, ionic phosphorus, or both;
- and
- a nucleation inhibitor in an amount effective to modulate ions of the crystallizable metals, reducing cytotoxic effects on the living cells by substantially controlling against spontaneous precipitation;
- and
- exposing the 3D cell-encapsulated matrix to the mineralizing solution for a period to achieve a selected mineralization level.
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Dependencies are inferred from the claim text.