Mutated Influenza Virus, Pharmaceutical Composition, and Use
AssigneesNingbo Institute of Marine Medicine, Peking University, Peking University
InventorsDemin ZHOU, Dezhong JI, Yuanjie ZHANG
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Abstract
The present invention belongs to the field of medicines and relates to a mutated influenza virus, a pharmaceutical composition, and use. Specifically, the present invention relates to a replication-defective influenza virus, a pharmaceutical composition, and use. More specifically, the present invention relates to a mutated influenza virus, wherein nucleic acid encoding HA protein and/or nucleic acid encoding NA protein of the influenza virus comprises one or more UAG codons. The replication-defective influenza virus or the pharmaceutical composition of the present invention can effectively treat or prevent tumors and has good application prospects.
Claims25
A mutated influenza virus, which comprises a nucleic acid encoding HA protein and/or a nucleic acid encoding NA protein, wherein the nucleic acid encoding HA protein and/or the nucleic acid encoding NA protein contains one or more UAG codons.
3-9. (canceled)
A mutated influenza virus, wherein the following sites are mutated to non-natural amino acids, such as NAEK:
- R52 site of PB1 protein, R266 site of PA protein, S53 site of HA protein and D101 site of NP protein;
- K33 site of PB2 protein, R266 site of PA protein, S53 site of HA protein and D101 site of NP protein;
- or
- K33 site of PB2 protein, R52 site of PB1 protein, S53 site of HA protein and D101 site of NP protein.
-16. (canceled)
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A plasmid-based influenza virus reverse genetics system, comprising:
- (1) 8 recombinant plasmids containing PB2 gene, PB1 gene, PA gene, HA gene, NP gene, NA gene, M gene and NS gene, respectively, and a vector, and
- (2) 4 additional recombinant plasmids containing PB2 gene, PB1 gene, PA gene and NP gene, respectively, and a vector;
- wherein the vector used for the recombinant plasmid in (2) is different from the vector used for the recombinant plasmid in (1);
- and the PB2 gene, PB1 gene, PA gene and NP gene in (2) are wild type;
- wherein, the HA gene and/or NA gene contain one or more TAG codons.
-29. (canceled)
Dependencies are inferred from the claim text.