US 2026/0061005 A1 · SINGLE-CHAIN FRAGMENT VARIABLE TARGETING HUMAN PDGFR-BETA AND USE THEREOF IN CAR-T CELL IMMUNOTHERAPY

Abstract

The present invention belongs to the technical fields of biomedicine and molecular biology, and particularly relates to a single-chain fragment variable (scFv) targeting human platelet-derived growth factor receptor (PDGFR)-β and use thereof in chimeric antigen receptor (CAR)-T cell immunotherapy. In the present invention, a scFv sequence targeting a human-derived PDGFRβ antigen is first obtained by immunizing a mouse, and then a second-generation CAR is constructed based on this, and additionally a CAR-T cell is obtained via lentivirus infection. The CAR-T cell can effectively kill a PDGFRβ antigen-positive 293T cell. The present invention provides a brand-new idea for eliminating PDGFRβ-positive cells to treat chronic kidney diseases, chronic liver diseases, cardiovascular diseases and various tumor diseases including various organ fibrosis, and has extremely attractive further development value and application prospects.

Claims18

Dependent claims

A single-chain fragment variable (scFv) targeting human platelet-derived growth factor receptor β (PDGFR-beta or PDGFRβ), comprising a scFv heavy chain VH and a scFv light chain VL;

wherein an amino acid sequence of the scFv heavy chain VH is as shown in SEQ ID NO:
1;
and an amino acid sequence of the scFv light chain VL is as shown in SEQ ID NO:
2.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

19/343,039Filed 29 Sep 2025

Priority

CN 202410952787516 Jul 2024

Published

5 Mar 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification24 codes

A61K 35/17
Blood; Artificial blood: lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
A61K 40/11
Characterised by the cell type used: t-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer…
A61K 40/31
Characterised by the recombinant expression of specific molecules in the cells of the immune system: chimeric antigen receptors [CAR]
A61K 40/4203
Receptors, cell surface antigens or cell surface determinants: receptors for growth factors

Single-chain fragment variable targeting human pdgfr-beta and use thereof in car-t cell immunotherapy

Life of this patent

Single-chain fragment variable targeting human pdgfr-beta and use thereof in car-t cell immunotherapy

Life of this patent

Abstract

Claims18