Platelet-rich plasma compositions, preparation, and uses thereof

A platelet-rich plasma (PRP) composition, comprising primed platelets and plasma, wherein:

• at least 40%, of the platelets in the composition are primed, but not activated;
• platelet-rich plasma (PRP) is a composition comprising plasma with a platelet count above that of peripheral blood;
• and
• primed platelets exhibit two or more of the following properties or markers:
• externalized phosphatidylserine, intact granules, negative for p-selectin on the platelet surface, retain discoid shape, and do not aggregate upon swirling or light agitation, where if the property is lack of aggregation upon swirling or light agitation, a second property of externalized phosphatidylserine, intact granules, negative for p-selectin on the platelet surface, and retention of discoid shape.