A computer-implemented method for providing fluorescent compound recommendations for fluorescent marking

A data processing system comprising at least one processor configured to:

• receive digital representations of potential fluorescent compounds for fluorescent marking;
• receive apparatus-related parameters related to any of light source emission spectra and wavelength-dependency of detection;
• provide respective spillover coefficients between respective pairs of the potential fluorescent compounds to obtain a set of spillover coefficients, wherein the respective spillover coefficients are calculated based on the apparatus-related parameters and are indicative of measurement spillover among the respective pairs of the potential fluorescent compounds;
• identify minimum coefficients, the minimum coefficients constituting a subset of the set of spillover coefficients, each respective coefficient of the minimum coefficients being identified based on that respective coefficient being lower than other coefficients of the set of spillover coefficients;
• identify a subset of the potential fluorescent compounds, wherein each pair of compounds within the subset of the potential fluorescent compounds has a spillover coefficient among said minimum coefficients;
• and
• provide at least some compounds of said subset of potential fluorescent compounds as fluorescent compound recommendations.

A computer program comprising instructions which, when the program is executed by a computer, cause the computer to carry out the steps of:

• receiving digital representations of potential fluorescent compounds for fluorescent marking;
• receiving apparatus-related parameters related to any of light source emission spectra and wavelength-dependency of detection;
• providing respective spillover coefficients between respective pairs of the potential fluorescent compounds to obtain a set of spillover coefficients, wherein the respective spillover coefficients are calculated based on the apparatus-related parameters and are indicative of measurement spillover among the respective pairs of the potential fluorescent compounds;
• identifying minimum coefficients, the minimum coefficients constituting a subset of the set of spillover coefficients, each respective coefficient of the minimum coefficients being identified based on that respective coefficient being lower than other coefficients of the set of spillover coefficients;
• identifying a subset of the potential fluorescent compounds, wherein each pair of compounds within the subset of the potential fluorescent compounds has a spillover coefficient among said minimum coefficients;
• and
• providing at least some compounds of said subset of potential fluorescent compounds as fluorescent compound recommendations.