Compositions, Devices, Systems, and Methods for Using a Nanopore
AssigneeThe Regents of the University of California
InventorsMark A. Akeson, Roger Jinteh Arrigo Chen
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Abstract
Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
Claims26
An apparatus comprising a nanopore device further comprising:
- a film separating a cis compartment from a trans compartment, the cis compartment comprising one or more polymers,
- a nanopore provided in the film, wherein the nanopore is dimensioned for passing the one or more polymers from the cis compartment to the trans compartment,
- electrodes arranged to apply an electric field between the cis compartment and the trans compartment,
- a measurement system configured and arranged to measure a transport property of the nanopore;
- and
- a finite state machine configured to control passage of the one or more polymers through the nanopore.
An apparatus comprising a nanopore device further comprising:
- a film separating a cis compartment from a trans compartment, the cis compartment comprising one or more polymers,
- a nanopore provided in the film, wherein the nanopore is dimensioned for passing the one or more polymers therethrough,
- electrodes arranged to apply an electric field between the cis compartment and the trans compartment,
- a measurement system configured and arranged to measure a transport property of the nanopore;
- and
- a processor configured to control passage of the one or more polymers through the nanopore.
Dependencies are inferred from the claim text.