Hairpin internal control for isothermal nucleic acid amplification

Abstract

Disclosed herein include methods, compositions, and kits for monitoring an amplification reaction. Some embodiments include a quality control template comprising a 5′ subdomain, a 3′ subdomain, and a loop domain situated between the 5′ subdomain and the 3′ subdomain. Intramolecular nucleotide base pairing between the 5′ subdomain and the 3′ subdomain are capable of forming a paired stem domain. A quality control primer can be capable of hybridizing to at least a portion of the 3′ subdomain. Subjecting the quality control template and the quality control primer to an amplification reaction can generate a first quality control product. The method can comprise detecting the first quality control product.

Dependencies are inferred from the claim text.