Archaeal polymerase amplification
AssigneeBecton, Dickinson and Company
InventorJean-Sebastien Cote
Life of this patent
Estimating the term…
Abstract
Disclosed herein include methods, compositions, and kits for detecting a plurality of nucleic acid sequences. The method can comprise amplifying a first nucleic acid sequence and a second nucleic acid sequence in an amplification reaction mixture, thereby generating a first nucleic acid amplification product and a second nucleic acid amplification product. The method can comprise detecting in the same optic channel the first nucleic acid amplification product and the second nucleic acid amplification product with a first signal-generating oligonucleotide and a second signal-generating oligonucleotide, respectively.
Claims100
A method for detecting a plurality of nucleic acid sequences, comprising:
- amplifying a first nucleic acid sequence and a second nucleic acid sequence in a amplification reaction mixture, thereby generating a first nucleic acid amplification product and a second nucleic acid amplification product, respectively;
- and
- detecting in the same optic channel the first nucleic acid amplification product and the second nucleic acid amplification product with a first signal-generating oligonucleotide and a second signal-generating oligonucleotide, respectively,
- wherein the first signal-generating oligonucleotide and the second signal-generating oligonucleotide each comprise a label, and
- wherein the detecting comprises detecting the signal of the label of the first signal-generating oligonucleotide and the second signal-generating oligonucleotide before the amplifying, during the amplifying, after the amplifying, or any combination thereof.
Dependencies are inferred from the claim text.