Compositions and methods for increasing the amino acid and protein content in storage organs of plants
AssigneePolopo Ltd.
InventorsRaya LIBERMAN-ALONI, Maya SAPIR-MIR
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Abstract
The present invention provides methods and compositions for increasing amino acids content and proteins in plant storage organs. In particular, the present invention relates to transgenic Solarium tuberosum plants overexpressing amino acid permease (AAP) and/or lysin histidine transporter (LHT), the plants store high amount of essential amino acids and capable of producing high amount of heterologous proteins in the tubers.
Claims25
A tuberous plant comprising at least one cell having an enhanced expression and/or activity of at least one amino acid permease (AAP) and/or at least one Lysin histidine like transporter (LHT), compared to the expression and/or activity in a corresponding control plant, and having:
- (1) an increased amount of at least one free amino acid in the plant tuber, and/or (2) an increased amount of at least one protein in said tuber;
- compared to the amount in the corresponding control plant.
9-11. (canceled)
(canceled)
22-24. (canceled)
A method of increasing the content of at least one amino acid and/or a protein in a plant tuber, the method comprising enhancing the expression and/or activity of AAP and/or LHT in the plant relative to a control plant.
26-28. (canceled)
A plant tuber comprising at least one exogenous polynucleotide encoding AAP and/or at least one exogenous polynucleotide encoding LHT or an active fragment thereof, wherein the plant tuber comprises an increased amount of at least one essential amino acid and/or total proteins compared to the amount in a corresponding plant tuber devoid of the at least one exogenous polynucleotide.
31-33. (canceled)
Dependencies are inferred from the claim text.