Scaffold materials for artificial dermal layer
AssigneeVitroLabs Inc
InventorsGabriel MICKEL, Rui TANG, Craig HALBERSTADT, Ingvar HELGASON
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Abstract
Disclosed herein are compositions for use as scaffold materials for artificial dermal layers. Disclosed herein are methods for the manufacture and processing of scaffold materials for artificial dermal layers. Disclosed herein are scaffold materials for use supporting immortalized fibroblasts.
Claims147
A composition comprising a scaffold in contact with an extracellular matrix comprising a collagen, wherein the scaffold comprises a non-woven, needle-punched material.
A composition comprising a scaffold in contact with an extracellular matrix comprising a collagen, wherein the scaffold comprises a three-dimensional woven material.
A composition comprising a scaffold in contact with an extracellular matrix comprising a collagen, wherein the scaffold comprises a polycaprolactone (PCL), a polylactic acid (PLA), a polylactic and polyglycolic acid (PLGA), a polyethylene terephthalate (PET), a nylon, a polyethylene (PE), a polyethylene furanoate (PEF), a polypropylene (PP), a polyvinyl alcohol (PVA), a cotton, a bast fiber, a viscose, a modal, a lyocell, a plant based protein fiber, a biobased material, a viscose, a cellulose, an alginate fiber, a thermoplastic starch, or any combination thereof.
A composition comprising an at least partially coated scaffold in contact with an extracellular matrix comprising a collagen, wherein the scaffold is at least partially coated with a coating comprising a matrigel, a vitronectin, a fibronectin, a protein extract from a soy, a protein extract from a pea, a protein extract from a corn, a peptide generated from synthesis, an RNA-binding glycine-rich (RBG) protein, a polylysine, a synthetic protein, an RGD peptide, a polylysine, a polyarginine, a polyornithine, a recombinant protein, an oligomer, a polymer, or any combination thereof.
A composition comprising a dissolvable scaffold in contact with an extracellular matrix comprising a collagen, wherein the scaffold is in contact with a solvent that can dissolve the scaffold.
A method comprising:
- a) seeding isolated animal fibroblasts or fibroblast-like cells onto a scaffold to form a cell layer on a scaffold, wherein the scaffold comprises a polycaprolactone (PCL), a polylactic acid (PLA), a polylactic and polyglycolic acid (PLGA), a polyethylene terephthalate (PET), a Nylon, a polyethylene (PE), a polyethylene furanoate (PEF), a polypropylene (PP), a polyvinyl alcohol (PVA), a cotton, a bast fiber, a viscose, a modal, a lyocell, a plant based protein fiber, an alginate fiber, a thermoplastic starch, or any combination thereof,
- b) growing the cells to produce a composition comprising an extracellular matrix;
- and
- c) tanning the composition comprising an extracellular matrix to form a cruelty-free leather.
Dependencies are inferred from the claim text.