Use of virus-like particles in vaccination
AssigneeThe Chinese University of Hong Kong
InventorsYee Yan Christine WAI, Ting Fan LEUNG, Yat Hin Nicki LEUNG
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Abstract
This invention provides a virus-like particle (VLP) that is formed by a protein of a viral origin (such as from a Hepatitis B virus) and derivatized by way of an isopeptide bond formed between two partner peptides (such as a SpyCatcher/SpyTag connection) to present on the VLP surface one or more antigenic epitopes of a target antigen. Also provided are methods of making the VLP, compositions comprising the VLP, as well as applications of the VLP and the compositions for modulating a recipient's immune response to the target antigen, including immunization against the antigen or desensitization to the antigen.
Claims25
A method for making an antigen epitope-presenting virus-like particle (VLP), comprising:
- (1) forming a VLP with a modified viral protein comprising at least a portion of the viral protein fused to a first partner peptide;
- and
- (2) reacting the VLP with a fusion antigenic peptide comprising an epitope of a target antigen fused with a second partner peptide to form an isopeptide bond between the first and second partner peptides and yield a VLP presenting the epitope of the target antigen.
Dependencies are inferred from the claim text.