Analyzer for automatically detecting and quantifying glutaraldehyde in biocide products

An analyzer for detecting and quantifying a glutaraldehyde (GLUT) composition in a water sample, the analyzer comprising:

• a peristaltic pump and a three-way valve operatively connected to the peristaltic pump, wherein the peristaltic pump is configured to draw in a water sample comprising a GLUT composition through the 3-way valve;
• a six-way valve operatively connected to the three-way valve via the peristaltic pump, wherein the six-way valve is configured to receive:
• a) the water sample from the 3-way valve via a first inlet and b) a bovine serum albumin-coated gold (Au-BSA) nanoclusters reagent via a second inlet;
• a debubbler operatively connected to the six-way valve and configured to receive a mixture of the water sample and the Au-BSA nanoclusters reagent and remove bubbles from the mixture;
• and
• a reaction coil operatively connected to the debubbler and configured to receive the water sample and the Au-BSA nanoclusters reagent, wherein the water sample and the Au-BSA nanoclusters reagent are mixed and reacted in the reaction coil;
• a fluorescence flow cell comprising a 375 nm light-emitting diode (LED), wherein the flow cell is configured to:
• receive the mixture from the reaction coil;
• measure a fluorescence intensity of the Au-BSA nanoclusters in the mixture at an emission wavelength of 675 nm, wherein the Au-BSA nanoclusters comprises a maximum excitation wavelength of approximately 375 nm and a maximum emission wavelength of approximately 675 nm;
• and
• determine a presence and concentration of the GLUT composition in the water sample based on a comparison between the measured fluorescence intensity of the Au-BSA nanoclusters at the emission wavelength of 675 nm with fluorescence intensity values of calibration samples comprising Au-BSA nanoclusters and known glutaraldehyde concentrations.