System for concentrating and capturing target material from liquid samples
OwnerScott Vincent Angus, Ada S. Cowaninventors
Life of this patent
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Abstract
A system and method for concentrating and capturing target material from a fluid sample, comprising directing a fluid sample containing a target material into a prefiltration cartridge, wherein the prefiltration cartridge further includes at least one prefilter, wherein the at least one prefilter further includes a plurality of stacked individual filters of predetermined diameters and pore sizes; directing the fluid sample from the prefiltration cartridge to a capture cartridge, wherein the capture cartridge further includes a capture filter disposed therein; capturing the target material on the capture filter; eluting the target material from the capture filter; and collecting the eluted target material.
Claims26
A method for concentrating and capturing target material from a fluid sample, comprising:
- (a) directing a fluid sample containing a target material into a prefiltration cartridge, wherein the prefiltration cartridge further includes at least one prefilter, and wherein the at least one prefilter further includes a plurality of stacked individual filters of predetermined diameters and pore sizes;
- (b) directing the fluid sample from the prefiltration cartridge to a capture cartridge, wherein the capture cartridge further includes a capture filter disposed therein;
- (c) capturing the target material on the capture filter;
- (d) eluting the target material from the capture filter;
- and
- (e) collecting the eluted target material.
A system for concentrating and capturing target material from a fluid sample, comprising:
- (a) a prefiltration cartridge, wherein the prefiltration cartridge further includes:
- (i) a sample reservoir configured to receive a fluid sample containing a target material;
- and
- (ii) at least one prefilter connected to or formed integrally with the sample reservoir, wherein the at least one prefilter further includes a plurality of stacked individual filters of predetermined diameters and pore sizes;
- (b) a capture cartridge in fluid communication with the prefiltration cartridge, wherein the capture cartridge further includes a capture filter disposed therein;
- (c) a first pump configured to pull the fluid sample through the prefiltration cartridge and into the capture cartridge, wherein target material from the fluid sample is captured on the capture filter;
- (d) an elution buffer reservoir in fluid communication with the capture cartridge, wherein the elution buffer reservoir is configured to store elution buffer;
- (e) a second pump in fluid communication with the elution buffer reservoir and the capture cartridge, wherein the second pump is configured to push the elution buffer into the capture cartridge and across the capture filter to release the captured target material therefrom;
- and
- (f) a collection reservoir in fluid communication with the capture cartridge, wherein the collection reservoir is configured to collect the target material eluted from the capture filter.
A system for concentrating and capturing target material from a fluid sample, comprising:
- (a) a prefiltration cartridge, wherein the prefiltration cartridge further includes:
- (i) a sample reservoir configured to receive a fluid sample containing a target material;
- and
- (ii) at least one prefilter connected to or formed integrally with the sample reservoir, wherein the at least one prefilter further includes a plurality of stacked individual filters of predetermined diameters and pore sizes;
- (b) a filtrate reservoir in fluid communication with the prefiltration cartridge, wherein the filtrate reservoir is configured to receive filtered fluid sample containing the target material, and wherein the filtrate reservoir further includers a weight sensing device or fluid flow sensing device;
- (c) a capture cartridge in fluid communication with the filtrate reservoir, wherein the capture cartridge further includes a capture filter disposed therein;
- (d) a waste reservoir in fluid communication with the capture cartridge, wherein the waste reservoir is configured to receive excess fluid from the capture cartridge;
- (e) a first pump configured to pull the fluid sample through the prefiltration cartridge into the filtrate reservoir and the into the capture cartridge, wherein target material from the filtered fluid sample is captured on the capture filter;
- (f) an elution buffer reservoir in fluid communication with the capture cartridge, wherein the elution buffer reservoir is configured to store elution buffer;
- (g) a second pump in fluid communication with the elution buffer reservoir and the capture cartridge, wherein the second pump is configured to push the elution buffer into the capture cartridge and across the capture filter to release the captured target material therefrom;
- and
- (f) a collection reservoir in fluid communication with the capture cartridge, wherein the collection reservoir is configured to collect the target material eluted from the capture filter.
Dependencies are inferred from the claim text.