Methods and products for transfecting cells
AssigneeFactor Bioscience Inc.
InventorsMatthew ANGEL, Christopher ROHDE
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Abstract
The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.
Claims61
A synthetic RNA molecule comprising three or more non-canonical nucleotides that each include one or more substitutions from the following:
- pyrimidine position 2C, pyrimidine position 4C, pyrimidine position 5C, purine position 6C, purine position 7N, and purine position 8C.
A synthetic RNA molecule that:
- a. comprises a non-canonical nucleotide, and
- b. encodes a gene-editing protein.
A therapeutic composition comprising a synthetic RNA molecule that encodes a gene-editing protein and a transfection reagent.
A method for transfecting a cell with a nucleic acid comprising:
- a. contacting the cell with a medium containing hydrocortisone and/or albumin, wherein the albumin is treated with an ion-exchange resin or charcoal, and
- b. contacting the cell with the nucleic acid.
A medium comprising albumin, wherein the albumin is:
- a. recombinant, and
- b. treated with an ion-exchange resin or charcoal.
A kit comprising:
- a. hydrocortisone and/or albumin, wherein the albumin is treated with an ion-exchange resin or charcoal, and
- b. a synthetic RNA molecule.
A nucleic acid transfection-reagent complex comprising a nucleic acid and a transfection reagent, wherein the nucleic acid transfection-reagent complex is solidified by cooling.
A system for transfecting cells comprising a means for contacting cells with a transfection medium and a means for contacting the cells with nucleic acid transfection-reagent complexes.
Dependencies are inferred from the claim text.