Methods for purification of messenger rna
OwnerFrank DeRosa, Michael Heartlein +4inventors
InventorsFrank DeRosa, Michael Heartlein, Jonathan Abysalh, Daniel Crawford, Anusha Dias, Shrirang Karve
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Abstract
The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
Claims34
A method for purifying mRNA, comprising steps of:
- providing a suspension comprising precipitated mRNA;
- and
- centrifuging the suspension in a centrifuge comprising a porous substrate such that the precipitated mRNA is captured on the porous substrate, thereby purifying contaminants from the mRNA.
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A composition comprising dried purified mRNA, wherein said mRNA is obtained by a method comprising
- providing a suspension comprising precipitated mRNA;
- centrifuging the suspension in a centrifuge comprising a porous substrate such that the precipitated mRNA is captured on the porous substrate, thereby purifying contaminants from the mRNA;
- washing the purified mRNA composition with a solvent;
- and
- drying the captured mRNA.
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Dependencies are inferred from the claim text.