Method of treating subjects with acute liver failure using extracorporeal bioengineered dual-cell liver regeneration system

Abstract

A method of treating a subject with acute liver failure is disclosed. The method involves including an EBDLR system into the subject's blood circuit and passing blood through the EBDLR system, where the blood is continuously taken from the subject. Further, the method includes separating the blood into a plasma component and remainder using a plasma separator of the EBDLR system and then passing the plasma component into a bio purifier having a plurality of layers. Each layer may include hepatocytes on a first side of a membrane in a first channel, and endothelial cells on a second side of the membrane in a second channel. Furthermore, the method includes splitting the plasma component into the first channel and the second channel of the plurality of layers to purify the plasma component, restoring the blood by combining the purified plasma component and the remainder, and continuously returning the restored blood into the subject.

Dependencies are inferred from the claim text.