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US 2026/0043809 A1Published application
Affinity capture reagents for mass spectrometry
AssigneeOxford University Innovation Limited
InventorsCarol ROBINSON, Corinne LUTOMSKI, Tarick EL-BABA
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Abstract
The invention relates to a peptide mass tag for recovery and identification of target proteins by mass spectrometry. The invention further relates to fusion polypeptides including the peptide mass tag and a method of analysing a target protein comprising use of the peptide mass tag.
Claims23
A peptide mass tag comprising a dipeptide with fragmentation propensity and a binding moiety.
A method of analysing a target protein, the method comprising:
- (i) providing
- a sample comprising the target protein, and
- a polypeptide comprising an affinity ligand for the target protein fused to a peptide mass tag, said peptide mass tag comprising a dipeptide with fragmentation propensity;
- (ii) exposing the sample to the polypeptide to bind the affinity ligand to the target protein and thus to produce a first composition comprising a polypeptide complex comprising the polypeptide in complex with the target protein;
- (iii) exposing the first composition to a capture agent, to reversibly bind the polypeptide complex to the capture agent;
- (iv) recovering the polypeptide complex from the capture agent, to provide a second composition comprising the polypeptide complex;
- (v) ionising the second composition to produce a tagged gas-phase ion, the tagged gas-phase ion comprising the polypeptide complex;
- and
- (vi) m/z analysing and detecting the tagged gas-phase ion, and/or an ion derived therefrom.
6 more elements
23-25. (canceled)
Dependencies are inferred from the claim text.