Method of Modulating Production of Secondary Metabolites
OwnerDillon Wei Peng Tay, Yee Hwee Lim +3inventors
InventorsDillon Wei Peng Tay, Yee Hwee Lim, Fong Tian Wong, Siew Bee Ng, Yoganathan Kanagasundaram
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Abstract
The invention relates to a method of modulating production of a metabolite in an actinomycetes bacterium comprising increasing the expression or activity of the Sporulation and Antibiotic Production Related gene A (SarA. SCO4069) polypeptide to modulate production of the metabolite in the actinomycetes bacterium. In one embodiment, the metabolite is valinomycin, daidzein, fluvirucin, demethyllydicamycin, or TPU-0037 analogues A. C and D.
Claims13
A method of modulating production of a metabolite in an actinomycetes bacterium, the method comprising increasing the expression or activity of the Sporulation and Antibiotic Production Related gene A (SarA) polypeptide to modulate production of the metabolite in the actinomycetes bacterium.
A method of screening for a metabolite produced by an actinomycetes bacterium, the method comprising a) increasing the expression or activity of the Sporulation and Antibiotic Production Related gene A (SarA) polypeptide in an actinomycetes bacterium to modulate production of the metabolite in the actinomycetes bacterium;
- and b) screening for production of the metabolite in the actinomycetes bacterium.
A method of preparing a drug compound library, the method comprising a) increasing the expression or activity of the Sporulation and Antibiotic Production Related gene A (SarA) polypeptide in an actinomycetes bacterium to modulate production of metabolites in the actinomycetes bacterium;
- and b) isolating metabolites from the actinomycetes bacterium to prepare the drug compound library.
13-15. (canceled)
Dependencies are inferred from the claim text.