Stabilized protein production process using bacillus host cells with salt feed

Abstract

The present invention relates to the field of industrial fermentation and protein production. In particular, it relates to a method for producing a protein of interest in a fermentation medium comprising the following steps a) inoculating a fermentation medium with a Bacillus host cell comprising a gene encoding a protein of interest under the control of a promoter; b) cultivating the Bacillus host cell in the fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, c) adding sulfate to the fermentation medium to reach a concentration of at least 20 mM of sulfate in the fermentation medium; and d) allowing the protein of interest to precipitate and/or crystallize during cultivation. Further contemplated is the use of a high sulfate concentration in a fermentation medium for producing a protein of interest in a Bacillus host cell and a crystallized protein of interest obtained by or obtainable by the method of the invention.

Dependencies are inferred from the claim text.