Back to results
US 2026/0043010 A1Published application
Enone reductases
AssigneeCodexis, Inc.
InventorsChristopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
Life of this patent
Estimating the term…
Abstract
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Claims24
An enone reductase polypeptide comprising an amino acid sequence that is selected from the group consisting of:
- (a) an amino acid sequence that is at least 80% identical to a reference sequence selected from the group consisting of SEQ ID NOs:
- 8, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216 and 217;
- (b) an amino acid sequence encoded by a nucleic acid sequence that hybridizes under stringent conditions to a reference nucleic acid sequence that encodes a reference sequence selected from the group consisting of SEQ ID NOs:
- 7, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, and 215, or a complementary sequence thereof, wherein the amino acid sequence comprises one or more residue differences as compared to the reference sequence at one or more residue positions selected from the group consisting of:
- residue corresponding to X5;
- residue corresponding to X10;
- residue corresponding to X28;
- residue corresponding to X38;
- residue corresponding to X40;
- residue corresponding to X44;
- residue corresponding to X75;
- residue corresponding to X83;
- residue corresponding to X117;
- residue corresponding to X119;
- residue corresponding to X122;
- residue corresponding to X124;
- residue corresponding to X147;
- residue corresponding to X148;
- residue corresponding to X153;
- residue corresponding to X154;
- residue corresponding to X179;
- residue corresponding to X209;
- residue corresponding to X240;
- residue corresponding to X248;
- residue corresponding to X251;
- residue corresponding to X252;
- residue corresponding to X255;
- residue corresponding to X259;
- residue corresponding to X294;
- residue corresponding to X295;
- residue corresponding to X296;
- residue corresponding to X297;
- residue corresponding to X302;
- residue corresponding to X304;
- residue corresponding to X305;
- residue corresponding to X311;
- residue corresponding to X315;
- residue corresponding to X330;
- residue corresponding to X333;
- residue corresponding to X339;
- residue corresponding to X358;
- residue corresponding to X369;
- residue corresponding to X376;
- residue corresponding to X379;
- residue corresponding to X384;
- residue corresponding to X386;
- residue corresponding to X397;
- residue corresponding to X399;
- and residue corresponding to X400.
46 more elements
7-14. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.