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US 2026/0043002 A1Published application
Generation of functional neutrophils and macrophages from induced pluripotent stem cells in chemically defined conditions using transient expression of etv2
OwnerIgor I. Slukvin, Vera Sergeyevna Brok Volchanskaya +4inventors
InventorsIgor I. Slukvin, Vera Sergeyevna Brok Volchanskaya, Kran Suknuntha, Anna Huttenlocher, David Alfred Bennin, Lucas Klemm
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Abstract
The present invention provides methods of producing in vitro derived neutrophils or macrophages in xenogen- and serum-free conditions from pluripotent stem cells and in vitro derived populations of neutrophils and macrophages. Methods of treatment using in vitro derived neutrophils or macrophages are also contemplated.
Claims5
A population of genetically modified CD16+ CD10-neutrophils made by the method comprising:
- (a) culturing CD144+hematoendothelial progenitor cells derived from pluripotent stem cells (PSCs), in xenogen- and serum-free medium comprising GM-CSF and FGF2 for a sufficient time to produce non-adherent myeloid progenitors;
- (b) isolating the non-adherent myeloid progenitors from the adherent cells;
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- (c) culturing the non-adherent myeloid progenitors in xenogen- and serum-free medium comprising G-CSF and retinoic acid agonist for a time sufficient to differentiate the myeloid progenitors into CD16+ CD10− neutrophils.
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