Separation and isolation of nucleic acids using affinity ligands bound to a solid surface

A method of separating a target macromolecule from a sample, comprising the steps of:

• a. selecting an affinity ligand that will bind to the target macromolecule;
• b. binding the affinity ligand to a surface to create a coupled surface-affinity ligand;
• c. placing the coupled surface-affinity ligand into a container;
• d. introducing the sample containing the target macromolecule to the coupled surface-affinity ligand and causing the coupled surface-affinity ligand to incubate with the sample for a residence time, wherein the target macromolecule binds to the affinity ligand;
• and
• e. separating the coupled surface-affinity ligand bound to the target macromolecule from the sample that has the target macromolecule removed therefrom;
• wherein the target macromolecule is DNA, wherein the affinity ligand is an intercalator, a minor groove binder, a major groove binder, or a combination thereof, and is a cyanine of benzothiazole-quinoline or benzoxazole-quinoline compound selected from:
• Sybr Green I (N′,N′-dimethyl-N-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N-propylpropane-1,3-diamine), Sybr Green II, Sybr Gold, and Sybr Safe ((Z)-4-((3-Methylbenzo[d]thiazol-2(3H)-ylidene)methyl)-1-propylquinolin-1-ium 4-methylbenzenesulfonate)),
• Thiazolorange-propyliodide (TO-I),
• 1-(3-aminopropyl)-4-{[3-methyl-2,3-dihydro-1,3-benzoxazol-2-ylidene]methyl}quinoline-1-ium chloride (YO—C3),
• 1-(3{[-3-methyl-2,3-dihydro-1,3-benzoxazol-2-ylidene]amino}propyl)-4-{[3-methyl-2,3-dihydro-1,3-benzoxazol-2-ylidene]methyl}quinoline-1-ium bromide (YO—C3-amidine),
• 1-(3-aminopropyl)-4-{[3-methyl-2,3-dihydro-1,3-benzothiazol-2-ylidene]methyl}quinoline-1-ium chloride (TO-C3)
• 1-(3{[-3-methyl-2,3-dihydro-1,3-benzothiazol-2-ylidene]amino}propyl)-4-{[3-methyl-2,3-dihydro-1,3-benzothiazol-2-ylidene]methyl}quinoline-1-ium bromide (TO-C3-amidine),
• or TO-C6

A method for isolating and removing DNA from a sample containing DNA and other nucleic acids, comprising the steps of:

• a. selecting an affinity ligand that will bind DNA;
• b. binding the affinity ligand to a surface to create a coupled surface-affinity ligand;
• c. placing the coupled surface-affinity ligand into a container;
• d. introducing the sample to the coupled surface-affinity ligand and causing the coupled surface-affinity ligand to incubate with the sample for a residence time, wherein the DNA binds to the affinity ligand;
• and
• e. separating the coupled surface-affinity ligand bound to the DNA from the sample that has the DNA removed therefrom,
• wherein the affinity ligand is an intercalator, a minor groove binder, a major groove binder, or a combination thereof, and is a cyanine of benzothiazole-quinoline or benzoxazole-quinoline compound selected from:
• Sybr Green I (N′,N′-dimethyl-N-[4-[(E)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]-1-phenylquinolin-1-ium-2-yl]-N-propylpropane-1,3-diamine), Sybr Green II, Sybr Gold, and Sybr Safe ((Z)-4-((3-Methylbenzo[d]thiazol-2(3H)-ylidene)methyl)-1-propylquinolin-1-ium 4-methylbenzenesulfonate)),
• Thiazolorange-propyliodide (TO-I),
• 1-(3-amninopropyl)-4-{[3-methyl-2,3-dihydro-1,3-benzoxazol-2-ylidene]methyl}quinoline-1-ium chloride (YO—C3),
• 1-(3{[-3-methyl-2,3-dihydro-1,3-benzoxazol-2-ylidene]amino}propyl)-4-{[3-methyl-2,3-dihydro-1,3-benzoxazol-2-ylidene]methyl}quinoline-1-ium bromide (YO—C3-amidine),
• 1-(3-aminopropyl)-4-{[3-methyl-2,3-dihydro-1,3-benzothiazol-2-ylidene]methyl}quinoline-1-ium chloride (TO-C3)
• 1-(3{[-3-methyl-2,3-dihydro-1,3-benzothiazol-2-ylidene]amino}propyl)-4-{[3-methyl-2,3-dihydro-1,3-benzothiazol-2-ylidene]methyl}quinoline-1-ium bromide (TO-C3-amidine),
• or TO-C6