Osteoinductive bone implant including harvested demineralized bone and bone morphogenetic proteins bound thereto obtained from the same donor

A method of making an osteoinductive implant, comprising the steps of:

• harvesting cancellous bone material from a donor;
• extracting at least one fragment from the harvested bone material;
• exposing the at least one fragment to a lysing agent, the lysing agent configured to release growth factors and bioactive materials from cellular material of the harvested bone material, the lysing agent further comprising acetic acid having a concentration of at least 1% and a molarity range of about 0.2M to 17M;
• rinsing the harvested bone material to further clean the at least one fragment or substantially neutralize pH of the at least one fragment;
• wherein the rinsing agent is at least one of:
• water, a saline, a peroxide, an alcohol, a crystalloid, an antibiotic solution, a preserving fluid, and a storage agent;
• dehydrating the harvested bone material by performing at least one of:
• evaporation, vacuum drying, and lyophilization;
• and
• packaging the harvested bone material by vacuum sealing;
• wherein the steps of rinsing the harvested bone material and substantially neutralizing the pH of the harvested bone material is conducted after at least a portion of the released growth factors and bioactive materials binds to the harvested bone material.