Methods for characterizing compositions comprising peanut antigens
AssigneeSanofi
InventorsKim Alving, Pierre deMontigny, Bing Huang Wang
Life of this patent
Estimating the term…
Abstract
Methods for determining an in vitro release profile of peanut allergens in a sample are provided. Methods for determining one or more signatures of peanut allergens in a sample are provided.
Claims24
A method for quantifying peanut allergens in composition, the method comprising:
- contacting a composition comprising one or more peanut allergens with an aqueous extract medium to produce an aqueous peanut extract;
- digesting the one or more peanut protein allergens present in the aqueous peanut extract to generate peanut protein allergen digest products;
- fragmenting the one or more peanut protein allergen digest products to generate peptide fragments;
- analyzing the peptide fragments and/or the peanut protein allergen digest products using a mass spectroscopic method to generate a peanut allergen signature;
- and
- determining an amount of the one or more peanut protein allergens in the composition by comparing the peanut allergen signature to a calibration curve,
- wherein the peanut allergen signature comprises allergen digest products from Ara h1, Ara h2 and Ara h6, and
- wherein the one or more peanut protein allergens in the aqueous peanut extract is present at a concentration of less than about 2 μg/ml.
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The method of claim 12, wherein the signature comprises allergen digest products having amino acid sequences selected from the group consisting of SEQ ID NO:17, SEQ ID NO:70, SEQ ID NO:177, SEQ ID NO:197, SEQ ID NO:198, SEQ ID NO:199, SEQ ID NO:200, SEQ ID NO:201, SEQ ID NO:236 and SEQ ID NO:237.
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A method for quantifying one or more peanut protein allergens in a composition, the method comprising:
- contacting a composition comprising one or more peanut allergens with an aqueous extract medium to produce an aqueous peanut extract;
- digesting the one or more peanut protein allergens present in the aqueous peanut extract to generate one or more peanut protein allergen digest products;
- fragmenting the one or more peanut protein allergen digest products using a mass spectroscopic method to generate peptide fragments;
- analyzing the peptide fragments and/or the one or more peanut protein allergen digest products to generate a peanut allergen signature comprising allergen digest products from Ara h1, Ara h2 and Ara h6;
- and
- determining an amount of the one or more peanut protein allergens in the composition by comparing the peanut allergen signature to a calibration curve.
A method for quantifying one or more peanut protein allergens in a composition, the method comprising:
- contacting a composition comprising one or more peanut allergens with an aqueous extract medium to produce an aqueous peanut extract;
- digesting the one or more peanut protein allergens present in the aqueous peanut extract to generate one or more peanut protein allergen digest products;
- fragmenting the one or more peanut protein allergen digest products to generate peptide fragments;
- analyzing the peptide fragments and/or the one or more peanut protein allergen digest products using a mass spectroscopic method to generate a peanut allergen signature comprising allergen digest products from Ara h1, Ara h2 and Ara h6;
- and
- determining an amount of the one or more peanut protein allergens in the composition by comparing the peanut allergen signature to a calibration curve,
- wherein the one or more peanut protein allergens in the aqueous peanut extract is present at a concentration of less than about 1.5 μg/ml.
Dependencies are inferred from the claim text.