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OwnerAli Ertürk, Hongcheng Mai +1inventors
InventorsAli Ertürk, Hongcheng Mai, Jie Luo
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The present invention relates to methods for large tissue labeling, clearing and/or imaging using labeling agents such as antibodies, and uses and products related thereto. The present invention includes inter alia methods for preparing an animal tissue for fluorescence microscopy, an animal tissue obtainable by said methods, methods for analyzing said animal tissues, and methods for the detection of metastases, for analyzing the biodistribution of a biopharmaceutical drug, and for analyzing the biodistribution of nanoparticles. The methods for preparing an animal tissue according to the present invention encompass whole-body labeling, clearing and imaging methods. The methods of the invention are advantageous in that they, for instance, allow the visualization of single cells within mammalian tissues, including whole mouse bodies or other large tissues, tumor metastases at the single cell level and of the distribution of biopharmaceutical drugs (e.g. the distribution of cancer-targeting therapeutic antibodies in whole animals such as intact mice) at single cell level in whole mouse using labeling agents such as antibodies.
A method for preparing an animal tissue for fluorescence microscopy, the method comprising:
The method according to any one of the preceding claims, wherein the fluorochrome-containing labeling agent is an antibody conjugated to said fluorochrome, said antibody being capable of binding to said target molecule, preferably wherein the antibody is an IgG, IgA, IgM, IgD or IgE.
The method according to any one of the preceding claims, further comprising, prior to labeling the target molecule in the fixed animal tissue with the labeling solution, the following step:
The method according to any one of the preceding steps, further comprising:
The method according to any one of the preceding claims comprising the following steps, in this order:
Dependencies are inferred from the claim text.