Micro-organism growth detection method, micro-organism acquisition method, micro-organism growth detection kit, micro-organism acquisition kit, and micro-organism growth evaluation method
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsAkira SASAKI, Yuri OTA, Naohiro NODA, Akiko YOKOTA, Tetsushi SUYAMA
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Abstract
A method of detecting growth of a microorganism includes: preparing, in a W/O emulsion, a droplet containing a microorganism and a dye whose fluorescence intensity changes due to interaction with a membrane component of the microorganism; culturing the microorganism in the droplet; and detecting growth of the microorganism based on the fluorescence intensity of the dye.
Claims8
A method of detecting growth of a microorganism, the method comprising:
- preparing, in a W/O emulsion, a droplet containing a microorganism and a styryl dye;
- culturing the microorganism in the droplet;
- and
- detecting growth of the microorganism based on fluorescence intensity of the styryl dye.
A method of obtaining a microorganism, the method comprising:
- preparing, in a W/O emulsion, a droplet containing a microorganism and a styryl dye;
- culturing the microorganism in the droplet;
- detecting growth of the microorganism based on fluorescence intensity of the styryl dye;
- and
- collecting the droplet in which the growth of the microorganism has been detected.
A kit for detecting growth of a microorganism in a droplet in a W/O emulsion, the kit comprising a styryl dye.
A kit for obtaining a microorganism in a droplet in a W/O emulsion, the kit comprising a styryl dye.
A method of evaluating growth of a microorganism, the method comprising using a styryl, as a reporter for growth of the microorganism in a droplet in a W/O emulsion.
Dependencies are inferred from the claim text.