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US 2026/0035735 A1Published application
De novo surface preparation and uses thereof
AssigneeElement Biosciences, Inc.
InventorsChunhong ZHOU, Sinan ARSLAN, Molly HE, Matthew KELLINGER, Hui Zhen MAH, Michael PREVITE, Lei SUN
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Abstract
Methods and formulations for preparing low non-specific binding surfaces are described, and the prepared surface can provide improved performance for nucleic acid detection and base calling applications. The surface provides more accurate nucleic acid detection, enhanced contrast to noise ratio, and better data collection.
Claims30
A method of performing nucleic acid sequence determination, the method comprising:
- a) providing a surface;
- wherein the surface comprises:
- i) a substrate;
- ii) at least one hydrophilic polymer coating layer on the substrate;
- and
- iii) a plurality of oligonucleotide molecules attached to at least one hydrophilic polymer coating layer;
- wherein the plurality of the attached oligonucleotide molecules are present with a surface density of at least 1000 molecules/μm2 and
- b) contacting a plurality of sample nucleic acid molecules with the plurality of attached oligonucleotide molecules, and
- c) performing at least a nucleotide binding or incorporation reaction, wherein the nucleotide is labeled with a detectable tag.
6 more elements
Dependencies are inferred from the claim text.