Method for liquefying agarose and producing neoagarotetraose/neoagarohexaose by using thermostable gh16b beta-agarase derived from novel agar-degrading bacterium

Abstract

The present invention relates to a method of producing neoagarotetraose (NA4) and neoagarohexaose (NA6) with high purity in large amounts by liquefaction of the substrate agarose using a thermostable GH16B β-agarase derived from a novel agarolytic bacterium Cellvibrio sp. KY-GH-1 deposited under accession number KCTC 13629BP. The method of producing neoagarotetraose (NA4) and neoagarohexaose (NA6) with high purity in large amounts by liquefaction of the substrate agarose using a thermostable GH16B β-agarase derived from a novel agarolytic bacterium Cellvibrio sp. KY-GH-1 deposited under accession number KCTC 13629BP according to the present invention may effectively produce NA4 and NA6 with high purity in large amounts by efficiently performing liquefaction of the substrate agarose, because the thermostable GH16B β-agarase exhibits excellent enzyme activity at a high temperature equal to or higher than the agarose gelling temperature and has long-term thermal stability even at high temperatures.

Dependencies are inferred from the claim text.