Methods and compositions for spatially-resolved single cell sequencing

Abstract

Provided herein, among other things, is a method for spatial single cell analysis. In some embodiments, the method may comprise: obtaining a cellular sample comprising nucleic acid molecules, binding spatial tags to the sample, removing any unbound or unreacted spatial tags, determining the location and identity of the spatial tag, dissociating the sample into single cells, performing single cell sequencing, determine the spatial barcode for each single cell, and assigning the single cell sequencing reads to its spatial location.

Dependencies are inferred from the claim text.