Soybean transgenic event mon87751 and methods for detection and use thereof
AssigneeMonsanto Technology LLC
InventorsKim A. Beazley, Wen C. Burns, Robert H. Cole, Ted C. MacRae, John A. Miklos, Lisa G. Ruschke, Kairong Tian, Liping Wei, Kunsheng Wu
Life of this patent
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Abstract
The invention provides a transgenic Glycine max event MON87751, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event MON87751. The invention also provides polynucleotides specific for event MON87751, plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides for event MON87751. The invention also provides methods related to event MON87751.
Claims25
A recombinant DNA molecule detectable in a sample containing soybean DNA, wherein the nucleotide sequence of said molecule is:
- a) selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:
- 3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:
- 8, SEQ ID NO:9, and SEQ ID NO:10;
- or
- b) a nucleotide sequence completely complementary to (a),
- wherein the presence of such DNA molecule is diagnostic for soybean event MON87751 DNA in said sample.
A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with soybean event MON87751 DNA in a sample, wherein detecting hybridization of said DNA molecule under said hybridization conditions is diagnostic for soybean event MON87751 DNA in said sample.
A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule that function as DNA primers when used together in an amplification reaction with a sample containing soybean event MON87751 template DNA to produce an amplicon diagnostic for said soybean event MON87751 DNA in said sample, wherein said amplicon comprises the nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:
- 3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:
- 10, SEQ ID NO:
- 17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:
- 20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:
- 25, and SEQ ID NO:26.
A soybean plant, soybean plant part, or soybean cell thereof comprising a recombinant polynucleotide molecule comprising the nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:
- 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:
- 10.
A method for protecting a soybean plant from insect infestation, wherein said method comprises providing in the diet of a lepidopteran pest of soybean an insecticidally effective amount of cells or tissue of the soybean plant comprising event MON87751.
A method of producing an insect resistant soybean plant comprising:
- a) sexually crossing two different soybean plants with at least one of the two different soybean plants comprising transgenic soybean event MON87751 DNA;
- b) sampling seed or tissue from progeny of said crossing;
- c) detecting the presence of a DNA segment diagnostic for soybean event MON87751 in the sample from step b) to identify progeny comprising soybean event MON87751 DNA;
- and
- d) selecting said progeny comprising soybean event MON87751 DNA, wherein said progeny is an insect resistant soybean plant comprising transgenic soybean event MON87751 DNA.
A soybean seed comprising a detectable amount of the nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:
- 4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:
- 7, SEQ ID NO:8, and SEQ ID NO:
- 10, or complete complements thereof.
A soybean plant, soybean plant part, or soybean seed thereof comprising DNA functional as a template when tested in a DNA amplification method producing an amplicon diagnostic for the presence of event MON87751 DNA.
A method of determining the zygosity of a soybean plant or soybean seed comprising event MON87751 comprising:
- a) contacting a sample comprising soybean DNA with a primer set capable of producing a first amplicon diagnostic for event MON87751 and a second amplicon diagnostic for native soybean genomic DNA not comprising event MON87751;
- i) performing a nucleic acid amplification reaction with said sample and said primer set;
- and
- ii) detecting in said nucleic acid amplification reaction said first amplicon diagnostic for event MON87751, or said second amplicon diagnostic for native soybean genomic DNA not comprising event MON87751;
- wherein the presence of only said first amplicon is diagnostic of a homozygous event MON87751 DNA in the sample, and the presence of both said first amplicon and said second amplicon is diagnostic of a soybean plant heterozygous for event MON87751 allele;
- or
- b) contacting a sample comprising soybean DNA with a probe set which contains at least a first probe that specifically hybridizes to event MON87751 DNA and at least a second probe that specifically hybridizes to soybean genomic DNA that was disrupted by insertion of the heterologous DNA of event MON87751 and does not hybridize to event MON87751 DNA,
- i) hybridizing the probe set with said sample under stringent hybridization conditions,
- wherein detecting hybridization of only said first probe under said hybridization conditions is diagnostic for a homozygous allele of event MON87751 DNA in said sample, and wherein detecting hybridization of both said first probe and said second probe under said hybridization conditions is diagnostic for a heterozygous allele of event MON87751 DNA in said sample.
Dependencies are inferred from the claim text.