Linked duplex target capture
AssigneeNcan Genomics, Inc.
InventorsJoel Pel, Andrea Marziali
Life of this patent
Estimating the term…
Abstract
The invention generally relates to sequencing library preparation methods. In certain embodiments, two or more template nucleic acids are joined together by a linking molecule, such as a PEG derivative. Identical copies of a nucleic acid fragment or both strands of a duplex fragment may be linked together. The linked nucleic acids are amplified, creating linked amplicons. Emulsion PCR with linked primers creates linked template nucleic acids for seeding sequencing clusters and errors can be readily identified by their presence on only one of the linked fragments.
Claims18
1-10. (canceled)
A method for preparing a sequencing library, the method comprising:
- ligating adapters comprising a universal priming site to nucleic acid fragments to form adapter-ligated fragments, wherein one adapter-ligated fragment comprises a region of interest;
- exposing the one adapter-ligated fragment to at least one linked target capture probe, the linked target capture probe comprising a universal primer portion connected to a target-specific probe portion;
- annealing (i) the universal primer portion to the universal priming site, and (ii) the target-specific probe portion to the region of interest;
- and
- amplifying or copying the one adapter-ligated fragment using at least the one linked target capture probe.
Dependencies are inferred from the claim text.