Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
OwnerJoseph M. BEECHEM, Gordon B. MILLS +5inventors
InventorsJoseph M. BEECHEM, Gordon B. MILLS, Charles WARREN, Chris MERRITT, Jaemyeong JUNG, Dwayne L. DUNAWAY, Scott CROWDER
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Abstract
The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein expression in a user-defined region of a tissue, user-defined cell, and/or user-defined subcellular structure within a cell.
Claims18
A probe, wherein the probe comprises a target binding domain and a signal oligonucleotide;
- wherein the target-binding domain is an antibody, a peptide, an aptamer, or a peptoid, and
- wherein the probe comprises a cleavable linker located between the target-binding domain and the signal oligonucleotide.
A kit comprising a plurality of probes, wherein each probe of the plurality of probes comprises a target binding domain and a signal oligonucleotide;
- and wherein the target-binding domain of each probe is independently an antibody, a peptide, an aptamer, or a peptoid, and
- wherein each probe comprises a cleavable linker located between the target-binding domain and the signal oligonucleotide.
Dependencies are inferred from the claim text.