Method for detecting mycobacterium tuberculosis
AssigneesKorea Atomic Energy Research Institute, Chungnam National University Hospital
InventorsJae Cheong LIM, Eun Ha CHO, So Young LEE, Sung Hee JUNG, Chae Uk CHUNG
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Abstract
The present invention relates to a method for detecting Mycobacterium tuberculosis, which can replace the conventional culture method that takes a long time of four to eight weeks to detect active tuberculosis, and which is a method for detecting active Mycobacterium tuberculosis by using isotopes on a sample of a patient's sputum or bronchoalveolar lavage fluid.
Claims5
A method for detecting Mycobacterium tuberculosis, comprising the steps of:
- (a) treating a biological sample isolated from a patient suspected of tuberculosis with N-acetyl-L-cysteine (NALC)—NaOH;
- (b) culturing the sample treated with N-acetyl-L-cysteine (NALC)—NaOH in a liquid medium for culturing Mycobacterium tuberculosis;
- (c) treating a culture product cultured in the liquid medium with urea (13C-urea) composed of a carbon isotope;
- and
- (d) measuring 13CO2 of the culture product.
A method for providing information for diagnosing tuberculosis, comprising the steps of:
- (a) treating a biological sample isolated from a patient suspected of tuberculosis with N-acetyl-L-cysteine (NALC)—NaOH;
- (b) culturing the sample treated with N-acetyl-L-cysteine (NALC)—NaOH in a liquid medium for culturing Mycobacterium tuberculosis;
- (c) treating a culture product cultured in the liquid medium with urea (13C-urea) composed of a carbon isotope;
- (d) measuring 13CO2 of the culture product;
- and
- (e) determining the patient as a tuberculosis patient if the level of 13CO2 is higher than a normal sample.
Dependencies are inferred from the claim text.