Enhanced recombination of genomic loci
AssigneeMonsanto Technology LLC
InventorsDavid G. CALDWELL, Ervin D. NAGY
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Abstract
The present disclosure provides methods to accelerate recombination at selected genomic loci, allowing recombination to occur, and selecting events with molecular variation within the selected loci. The accelerated recombination generates novel variations in gene clusters that are present in the plant or mammalian genomes.
Claims41
A method of generating a new gene array, comprising contacting a cell with a first site-specific genome modification enzyme that introduces a genome modification in at least one target sequence in a first gene array, thereby inducing recombination with a second gene array, and selecting at least one progeny comprising a new array of genes.
A method of providing a plant with improved disease resistance, comprising:
- a. providing to one or more plant cells a site-specific genome modification enzyme that introduces a genome modification at least one target sequence in a disease resistance locus;
- b. screening for asymmetric recombination between disease-resistance loci on homologous chromosomes to identify plant cells comprising a recombinant disease resistance locus;
- c. testing plants obtained from the plant cells identified in step (b) and their progeny for improved disease resistance;
- and
- d. selecting the plant with improved disease resistance.
A method of generating a plant from an inbred line with an altered disease resistance locus compared to a disease resistance locus in a parental genome, comprising providing a site-specific genome modification enzyme to a plant cell, wherein the site-specific genome modification enzyme introduces a genome modification at least one target sequence in one or more disease resistance loci thereby inducing asymmetric recombination between the disease resistance locus in the first parental genome and the disease resistance locus in the second parental genome and growing the plant with the altered disease resistance locus from the plant cell.
A plant, plant cell or a seed of a plant produced by the method of any one of claims 1-41.
Dependencies are inferred from the claim text.