Back to results
US 2026/0028601 A1Published application
Recombinase polymerase amplification
AssigneeAbbott Diagnostics Scarborough, Inc.
InventorsOlaf Piepenburg, Niall A. Armes, Matthew James David Parker
Life of this patent
Estimating the term…
Abstract
The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.
Claims21
1-25. (canceled)
A composition comprising:
- a mutant UvsX protein comprising an amino acid substitution of a histidine in a Walker A box amino acid sequence;
- a single stranded binding protein;
- recombinase loading agent;
- a strand displacing polymerase;
- and
- a crowding agent.
3 more elements
Dependencies are inferred from the claim text.