Method for producing high yield and high quality recombinant adeno-associated virus
AssigneeAavatar Therapeutics Co., Ltd.
InventorsSeunghee CHO, Sunhak HEO, Hyeonjae CHOI
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Abstract
The present specification provides a method for producing a recombinant adeno-associated virus (rAAV) of a desired composition in a high yield and a high quality. The method for producing a recombinant adeno-associated virus disclosed in the present application is characterized by comprising a pre-culture process and an osmotic shock process. The pre-culture process and the osmotic shock process synergize with each other within the production method to produce recombinant adeno-associated virus in high yield and high quality.
Claims4
A method for producing a recombinant Adeno Associated Virus (rAAV), comprising:
- (a) preparing host cells, wherein the host cell comprises a vector encoding components of the rAAV, such that the host cells are capable of expressing the rAAV;
- (b) adding sugar, Nocodazole and M344 to a medium comprising the host cells;
- (c) culturing the host cells, wherein the culturing enables the expression of the rAAV within the host cells;
- (d) applying an osmotic shock by adding salt to the media comprising the host cells,
- wherein the osmotic shock facilitates the release of the rAAV from the host cells into the medium;
- and
- (e) obtaining the rAAV from the medium without lysing the host cells.
Dependencies are inferred from the claim text.