Bone augmentation utilizing muscle-derived progenitor compositions in biocompatible matrix, and treatments thereof

A method of improving at least one symptom associated with bone disease, defect or pathology in a mammalian subject in need thereof comprising:

• (a) isolating skeletal muscle cells from a mammal,
• (b) suspending the mammalian skeletal muscle cells in a first cell culture container for between 30 and 120 minutes;
• (c) decanting the media from the first cell culture container to a second cell culture container;
• (d) allowing the remaining cells in the media to attach to the walls of the second cell culture container;
• (e) isolating the cells from the walls of the second cell culture container, wherein the isolated cells are MDCs;
• (f) providing small intestine submucosa (SIS);
• (g) seeding the SIS with MDCs;
• and
• (h) administering the MDC seeded SIS to a bone suffering from the bone defect, disease or pathology of the mammalian subject;
• thereby, improving at least one symptom associated with bone disease, defect or pathology in a mammalian subject in need thereof.

A method of improving at least one symptom associated with bone disease, defect or pathology in a mammalian subject in need thereof comprising:

• (a) plating a suspension of skeletal muscle cells from mammalian skeletal muscle tissue in a first container to which fibroblast cells of the skeletal muscle cell suspension adhere, (b) re-plating non-adherent cells from step (a) in a second container, wherein the step of re-plating is after 15-20% of cells have adhered to the first container;
• (c) repeating step (b) at least once;
• and
• (d) isolating the non-adherent cells wherein the isolated cells are MDCs;
• (e) providing small intestine submucosa (SIS);
• (f) seeding the SIS with MDCs;
• and
• (g) administering the MDC seeded SIS to a bone suffering from the bone defect, disease or pathology of the mammalian subject;
• thereby, improving at least one symptom associated with bone disease, defect or pathology in the mammalian subject in need thereof.