Pantoic acid-producing recombinant microorganism and use thereof
AssigneesAnhui Huaheng Biotechnology Co., Ltd., Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsXueli Zhang, Henghua Guo, Pingping Liu, Dongzhu Zhang, Jinlei Tang, Chao Zhang
Life of this patent
Estimating the term…
Abstract
The present invention provides a genetically engineered pantoic acid-producing strain having or having an enhanced NADH-dependent acetohydroxy acid reductoisomerase, a method for producing the strain, a method for producing D-pantoic acid using the strain, and use thereof in production of D-pantoic acid.
Claims17
A genetically engineered pantoic acid-producing strain, having or having an enhanced NADH-dependent acetohydroxy acid reductoisomerase, wherein preferably, the NADH-dependent acetohydroxy acid reductoisomerase is derived from Thermacetogenium phaeum, and more preferably comprises an amino acid sequence shown in SEQ ID NO:
- 9 or an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher identity thereto and having NADH-dependent acetohydroxy acid reductoisomerase activity.
A method for producing a genetically engineered pantoic acid-producing strain, comprising:
- in Escherichia coli deposited in the China General Microbiological Culture Collection Center (CGMCC) in Beijing, China, with a deposit number CGMCC No. 21699, replacing an NADPH-dependent acetohydroxy acid reductoisomerase-encoding gene with a gene encoding an NADH-dependent acetohydroxy acid reductoisomerase,
- wherein preferably, the NADH-dependent acetohydroxy acid reductoisomerase is derived from a Thermacetogenium phaeum strain, more preferably, the NADH-dependent acetohydroxy acid reductoisomerase comprises an amino acid sequence shown in SEQ ID NO:
- 9 or an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher identity thereto and having NADH-dependent acetohydroxy acid reductoisomerase activity, and more preferably, the gene encoding the NADH-dependent acetohydroxy acid reductoisomerase comprises a nucleotide sequence shown in SEQ ID NO:
- 10 or a degenerate sequence thereof, or a nucleotide sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher identity thereto.
A method for improving D-pantoic acid production of a pantoic acid-producing strain, comprising:
- conferring or enhancing activity of an NADH-dependent acetohydroxy acid reductoisomerase in the pantoic acid-producing strain, wherein preferably, the NADH-dependent acetohydroxy acid reductoisomerase is derived from Thermacetogenium phaeum, and more preferably comprises an amino acid sequence shown in SEQ ID NO:
- 9 or an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or higher identity thereto and having NADH-dependent acetohydroxy acid reductoisomerase activity.
Dependencies are inferred from the claim text.