Method, enzyme composition, nucleic acid composition, and transgenic microorganism for producing isoprene glycol

An enzyme composition for producing isoprene glycol, comprising:

• a first group comprising:
• acetyl-CoA acetyltransferase (AtoB), 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase (MvaS), HMG-CoA dehydratase/methylcrotonyl-CoA (MC-CoA) hydratase (LiuC), and methylglutaconyl-CoA (MG-CoA) decarboxylase (AibA/AibB);
• and
• a second group or a third group, wherein the second group comprises:
• coenzyme A-acylating aldehyde dehydrogenase, and the third group comprises thioesterase (YciA) and carboxylic acid reductase (CAR).

A transgenic microorganism for producing isoprene glycol, comprising:

• an acetyl-CoA generation pathway, producing acetyl-CoA from a carbon source;
• a first stage pathway, generating 3-hydroxy-3-methylbutyryl-CoA (HMB-CoA) from the acetyl-CoA;
• a second (A) stage pathway or a second (B) stage pathway, wherein the second (A) stage pathway reduces the HMB-CoA to produce 3-methyl-3-hydroxybutyrylaldehyde (3-HMBA), and the second (B) stage pathway hydrolyzes the HMB-CoA to 3-hydroxy-3-methylbutyric acid (HMB) and then reduces the HMB to 3-HMBA;
• and
• a third stage pathway, reducing the 3-HMBA to isoprene glycol (ISPG).