Method of producing collagen

A method for producing nano collagen from an animal skin comprising:

• applying to said skin a chemical mixture and salt additives in a predefined ratio and incubating said skin for a predefined sufficient time to loosen attached wool or hair;
• extracting said collagen from said skin;
• and
• preparing collagen nanoparticles using nano-milling and liquid nitrogen,
• wherein said chemical solution is a mixture of an organic acid, sodium acetate and water in a predefined ratio and said salt additives are inorganic acid or metal fluorides.

A method for producing collagen from an animal skin comprising:

• washing a mammalian animal skin in detergent and sodium metabisulphite solutions;
• applying to said skin a chemical solution and salt additives and incubating said skin for sufficient time to loosen attached wool or hair;
• and
• extracting said collagen from said skin.

A method for producing collagen from an animal skin comprising:

• dewooling a mammalian pelt using a dewooling paint, said paint including comprising acetic acid, anhydrous sodium acetate, sodium fluoride and boric acid;
• applying to said skin a chemical solution, salt additive;
• incubating said skin for sufficient time to loosen attached wool or hair;
• and
• extracting said collagen from said skin.

A method of producing purified native collagen from an animal skin comprising:

• providing an animal skin;
• contacting said skin with chemical solution and salt additives for predetermined time to substantially digest the glycosaminoglycans present in the skin to produce a pelt;
• mincing said pelt and exposing to one or more protease(s) for a predetermined time and temperature to produce a preparation comprising soluble and dispersed collagen;
• centrifuging/paddle separation said preparation to isolate soluble native collagen from dispersed collagen;
• and
• precipitating said soluble native collagen with salt to produce purified collagen.

A method for producing hydrolysable collagen from an animal skin comprising:

• washing dewooled ovine pelts followed by mincing;
• digesting minced pelts with citric acid (0.8 g/kg pelt), at pH 2.5-3.5, for 3 to 20hours at approximately 50 degrees Celsius to obtain acid digested mince;
• treating the digested minced pelts with enzyme mix at a temperature of 800 C-1000 C for 5-20 minutes, wherein the enzyme mix is AFP plus bromelain in a ratio of 1:20;
• removing by filtration an insoluble material recovered after enzyme treatment;
• and
• spray drying the filtrate to form collagen powder.